Goat anti-Rabbit Ig Antibody (CypHer5E)

Catalog No. ABIN1742571

Quick Overview for Goat anti-Rabbit Ig Antibody (CypHer5E) (ABIN1742571)

Target: Ig

Fragment: Fab fragment

Clonality: Polyclonal

Conjugate: CypHer5E

Application: Immunocytochemistry (ICC)

Product Details

Reactivity: Rabbit

Host: Goat

Purification: affinity purified Fab fragments. Rabbit serum albumin was added for stabilization.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. Application Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µL for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay. ICC: image

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives.

Target Details

Target: Ig